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pIF21, a versatile donor of transposons Tn1, Tn5 and Tn7 for tagging and mobilizing cryptic and non-selectable
I M Feavers1, A J Fife, C R Harwood
1Department of Microbiology, Medical School, Newcastle upon Tyne, UK.
Letters in Applied Microbiology
|November 1, 1990
Abstract:
A plasmid, pIF21, has been constructed that is able to donate transposons Tn1, Tn5 and Tn7. The transposons are located on a temperature-sensitive derivative of the incP1 plasmid pRP1, which is transferable to a wide range of Gram-negative genera.