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Mathematical modelling and simulation of aqueous two-phase continuous protein extraction.
S L Mistry1, J A Asenjo, C A Zaror
1University of Reading, Biochemical Engineering Laboratory, England.
Bioseparation
|January 1, 1992
Summary
A new mathematical model optimizes continuous protein extraction using aqueous two-phase systems. This model predicts performance and aids in developing control strategies for stable, efficient bioprocessing.
Area of Science:
- Biochemical Engineering
- Process Modeling
- Separation Science
Background:
- Aqueous two-phase systems (ATPS) are effective for protein extraction.
- Continuous, steady-state operation of ATPS requires robust process understanding.
- Optimizing protein recovery and purity is crucial in biopharmaceutical manufacturing.
Purpose of the Study:
- To develop a mathematical model for continuous, steady-state ATPS protein extraction.
- To predict the performance and robustness of ATPS under various conditions.
- To provide a basis for implementing control strategies for process stability.
Main Methods:
- Development of a mathematical model based on steady-state mass balances.
- Incorporation of phase equilibrium data for component partitioning.
- Validation using experimental data from thaumatin separation in a PEG4000/Phosphate system.
Main Results:
- The model accurately describes thaumatin separation in a PEG4000/Phosphate ATPS.
- Demonstrated the effect of NaCl on protein partitioning between phases.
- Simulation results show sensitivity to key process parameters and their impact on performance.
Conclusions:
- The developed model can predict protein recovery yield and purity.
- The model aids in assessing process robustness and optimizing operating conditions.
- It provides a foundation for control strategy implementation to ensure process stability.