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Polymer fractionation in aqueous two-phase polymer systems.

H Hartounian1, S I Sandler

  • 1Department of Chemical Engineering, University of Delaware, Newark 19716.

Biotechnology Progress
|May 1, 1991
PubMed
Summary

Adding different molecular weights of poly(ethylene glycol) (PEG) to aqueous two-phase systems reduces high molecular weight PEG in the dextran-rich phase. This finding optimizes polymer partitioning and reduces the loss of ligated PEG in separations.

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Area of Science:

  • Polymer Chemistry
  • Separation Science
  • Biophysical Chemistry

Background:

  • Aqueous two-phase systems (ATPS) are widely used for bioseparations.
  • Understanding polymer partitioning in ATPS is crucial for optimizing separation efficiency.
  • Poly(ethylene glycol) (PEG) and dextran are common polymers used in ATPS.

Purpose of the Study:

  • To investigate the molecular weight partitioning of poly(ethylene glycol) (PEG) in PEG-dextran ATPS.
  • To determine if solution thermodynamic models can predict polymer partitioning behavior.
  • To improve aqueous two-phase separations, particularly those using affinity ligands.

Main Methods:

  • Experimental determination of PEG molecular weight partitioning in PEG-dextran ATPS.
  • Varying PEG molecular weights and concentrations in the feed.

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  • Utilizing solution thermodynamic models for predictive analysis.

    • Main Results:

    • Increasing PEG concentration in the feed reduces high molecular weight PEG concentration in the dextran-rich phase.
    • This effect can minimize the loss of expensive ligated PEG during affinity partitioning.
    • Experimental data showed good agreement with predictions from a solution thermodynamic model.

    Conclusions:

    • The study provides insights into controlling polymer partitioning in ATPS.
    • Findings can enhance the efficiency and reduce costs in affinity-based separations.
    • Solution thermodynamic models offer a viable approach for optimizing ATPS without extensive experimentation.