Avian scale development. XVI. Epidermal commitment to terminal differentiation is prior to definitive scale ridge...

Germinative cells of the scutate scale epidermis from 15-day embryos are committed to appendage-specific, beta stratum formation in association with a foreign dermis. Commitment precedes the time (17 days of development) at which beta strata are actually present in their site-specific locations along the outer surface of each scutate scale. This observation suggested the possibility that commitment to beta stratum formation might be occurring as the outer epidermal surface of each scutate scale first becomes established between 12 and 13 days of development. It is at this time that the scale epidermis loses its ability to participate in feather morphogenesis and cell proliferation becomes restricted to a true stratum basale. To examined the ability of the presumptive scutate scale epidermis to generate beta strata in the absence of the inductive scutate scale dermis, scutate scale epidermis from 11-, 12-, and 13-day embryos was recombined with 15-day reticulate scale dermis and grown for 7 or 9 days. The dermis of reticulate scales does not induce beta stratum formation, but it does support differentiation of a beta stratum by the determined 15-day scutate scale epidermis. Using immunohistological and biochemical analyses of beta-keratins, we find that each of these presumptive scutate scale epidermises is competent to generate appendage-specific beta strata in the absence of the scutate scale dermis. This determination is occurring prior to scale ridge morphogenesis and differentiation of the epidermis into the distinct outer and inner epidermal surfaces of the scale ridge. The restricted distribution of beta strata to the apical domes of individual reticulate-like scales demonstrates that the germinative cells of the committed epidermises are responding to patterned cues. This study also suggests that all basal cells of the presumptive scutate scale epidermis are initially endowed with the ability to generate cells that form a beta stratum.