Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Allogeneic tumor-specific cytotoxic T lymphocytes.

J M Redd1, A C Lagarde, C A Kruse

  • 1Barbara Davis Center for Childhood Diabetes, University of Colorado Health Sciences Center, Denver 80262.

Cancer Immunology, Immunotherapy : CII
|January 1, 1992
PubMed
Summary

Researchers developed cytotoxic T lymphocytes targeting an allogeneic brain tumor in rats. These tumor-specific T cells show promise for adoptive immunotherapy against brain tumors.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The potential for genetically altered microglia to influence glioma treatment.

CNS & neurological disorders drug targets·2013
Same author

Immunotherapy of malignant gliomas using autologous and allogeneic tissue cells.

Anti-cancer agents in medicinal chemistry·2010
Same author

X-ray microbeams: Tumor therapy and central nervous system research.

Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment·2007
Same author

Permanent, lowered HLA class I expression using lentivirus vectors with shRNA constructs: Averting cytotoxicity by alloreactive T lymphocytes.

Transplantation proceedings·2006
Same author

CD20 expression in normal canine B cells and in canine non-Hodgkin lymphoma.

Veterinary pathology·2005
Same author

Suicide gene therapy with Herpes simplex virus thymidine kinase and ganciclovir is enhanced with connexins to improve gap junctions and bystander effects.

Histology and histopathology·2003

Area of Science:

  • Immunology
  • Oncology
  • Neuroscience

Background:

  • Cytotoxic T lymphocytes (CTLs) are crucial for adaptive immunity.
  • Targeting brain tumors with immunotherapy remains challenging.
  • Allogeneic models are valuable for studying anti-tumor immune responses.

Purpose of the Study:

  • To investigate the development of CTLs specific for an allogeneic brain tumor.
  • To determine if CTLs generated against a tumor recognize normal alloantigens.
  • To explore the potential of tumor-specific CTLs for brain tumor immunotherapy.

Main Methods:

  • Utilized a rat model with DA strain rats and Fischer strain 9L gliosarcoma.
  • Stimulated DA cytotoxic T cell precursors with allogeneic 9L tumor cells.

Related Experiment Videos

  • Assessed CTL lysis of tumor targets versus normal lymphoid blast targets.
  • Depleted DA T cells of reactivity to Fischer haplotype (DA-F) to analyze specificity.

    • Main Results:

    • Generated CTLs that lysed the allogeneic 9L tumor but not normal Fischer lymphoid blasts.
    • Demonstrated that CTL response to the tumor did not require T cell precursors specific for normal Fischer alloantigens.
    • Confirmed that preferential tumor lysis was not due to higher antigen density on the tumor.
    • Showed that DA-F T cells retained reactivity to the 9L tumor, indicating tumor-specific recognition.

    Conclusions:

    • Developed cytotoxic T lymphocytes exhibit specificity for a determinant expressed exclusively by the tumor.
    • These tumor-specific CTLs represent a potential tool for in vivo adoptive immunotherapy of brain tumors.