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Characterization of a class 3 tyrosine kinase
1Biological Carcinogenesis and Development Program, Program Resources, Inc/DynCorp, Frederick, Maryland 21702.
Oncogene
|May 1, 1992
Summary
Researchers identified Janus Kinase 1 (JAK1) in human leukemia cells using PCR. Interferon-gamma increases JAK1 mRNA in monocytes, and JAK1 is mapped to chromosome 1.
Area of Science:
- Molecular Biology
- Human Genetics
- Cancer Research
Background:
- Leukemia cell lines can harbor unique tyrosine kinases.
- Class 3 tyrosine kinases are ubiquitously expressed and lack ligand-binding and SH2 domains.
Purpose of the Study:
- To identify unique tyrosine kinases in human leukemia cell lines.
- To characterize the class 3 tyrosine kinase JAK1.
Main Methods:
- Polymerase chain reaction (PCR) and degenerate oligonucleotide primers were used to create a cDNA library.
- Northern blot analysis was performed to assess mRNA levels.
- Human-hamster somatic cell hybrid panels and linkage mapping were employed for gene localization.
Main Results:
- A novel class 3 tyrosine kinase, identified as JAK1, was discovered in the AML-193 cell line.
- Interferon-gamma treatment increased steady-state mRNA levels of JAK1 in human monocytes.
- JAK1 was assigned to human chromosome 1.
Conclusions:
- JAK1 is a relevant tyrosine kinase in human leukemia.
- JAK1 expression is modulated by interferon-gamma.
- The genetic locus of JAK1 was determined on chromosome 1.