Related Experiment Video

Updated: May 4, 2026

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
07:24

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

8.4K

Purification and properties of polyol dehydrogenase from Cephalosporium chrysogenus

S Birken, M A Pisano

    Journal of Bacteriology
    |January 1, 1976
    PubMed

    More Related Videos

    Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition08:31

    Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

    Published on: October 3, 2018

    8.1K
    Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase10:14

    Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

    Published on: November 8, 2019

    5.7K

    Related Concept Videos

    Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide02:44

    Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide

    Alkenes are converted to 1,2-diols or glycols through a process called dihydroxylation. It involves the addition of two hydroxyl groups across the double bond with two different stereochemical approaches, namely anti and syn. Dihydroxylation using osmium tetroxide progresses with syn stereochemistry.
    11.1K
    Oxidation of Alkenes: Anti Dihydroxylation with Peroxy Acids02:04

    Oxidation of Alkenes: Anti Dihydroxylation with Peroxy Acids

    Diols are compounds with two hydroxyl groups. In addition to syn dihydroxylation, diols can also be synthesized through the process of anti dihydroxylation. The process involves treating an alkene with a peroxycarboxylic acid to form an epoxide. Epoxides are highly strained three-membered rings with oxygen and two carbons occupying the corners of an equilateral triangle. This step is followed by ring-opening of the epoxide in the presence of an aqueous acid to give a trans diol.
    6.1K
    Acid-Catalyzed Dehydration of Alcohols to Alkenes02:35

    Acid-Catalyzed Dehydration of Alcohols to Alkenes

    In a dehydration reaction, a hydroxyl group in an alcohol is eliminated along with the hydrogen from an adjacent carbon. Here, the products are an alkene and a molecule of water. Dehydration of alcohols is generally achieved by heating in the presence of an acid catalyst. While the dehydration of primary alcohols requires high temperatures and acid concentrations, secondary and tertiary alcohols can lose a water molecule under relatively mild conditions.
    22.3K
    Preparation of Diols and Pinacol Rearrangement01:57

    Preparation of Diols and Pinacol Rearrangement

    Compounds bearing two hydroxyl groups are known as diols. When the hydroxyl groups are located on adjacent carbon atoms, the diols are called vicinal diols or glycols. Under acidic conditions, vicinal diols undergo a specific reaction called pinacol rearrangement.
    The reaction begins with transferring a proton from the acid catalyst to one of the hydroxyl groups, producing an oxonium ion.
    3.2K
    ¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

    ¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

    When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
    1.3K
    Dehydration of Aldols to Enones: Acid-Catalyzed Aldol Condensation00:43

    Dehydration of Aldols to Enones: Acid-Catalyzed Aldol Condensation

    As shown in Figure 1, under acidic conditions, the β-hydroxy ketone undergoes dehydration via an E1 elimination reaction to form an enone.
    2.2K