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Updated: Sep 14, 2026

Quantitation of Endothelial Cell Adhesiveness In Vitro
Published on: June 18, 2015
Monocyte attachment to activated human vascular endothelium in vitro is mediated by leukocyte adhesion molecule-1
O Spertini1, F W Luscinskas, M A Gimbrone
1Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts.
Abstract:
The receptors that mediate monocyte adhesion to cytokine-stimulated endothelial monolayers were assessed using a nonstatic (rotating) cell-attachment assay. In this system, leukocyte adhesion molecule-1 (LAM-1) (L-selectin) mediated a major portion (87 +/- 15% at 37 degrees C) of monocyte attachment to activated endothelium. mAb blocking of endothelial leukocyte adhesion molecule-1 (41% inhibition), CD18 (36%), and vascular cell adhesion molecule-1 (25%) function had lesser effects on attachment. These results suggest that LAM-1 may serve an important role in monocyte attachment to endothelium at sites of inflammation.
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