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CD20 expression is increased on B lymphocytes from HIV-infected individuals
F J Staal1, M Roederer, J Bubp
1Department of Genetics, Stanford University School of Medicine, California 94305.
Journal of Acquired Immune Deficiency Syndromes
|January 1, 1992
Summary
CD20 expression on B cells increases with HIV disease progression. Intracellular glutathione levels become more varied and correlate with CD20, suggesting CD20 as a potential marker for monitoring HIV infection.
Area of Science:
- Immunology
- Virology
- Biochemistry
Background:
- Human Immunodeficiency Virus (HIV) infection impacts immune cells, including B lymphocytes.
- Understanding B cell alterations is crucial for managing HIV infection.
Purpose of the Study:
- To investigate the expression of the B cell marker CD20 in HIV-infected individuals.
- To explore the relationship between CD20 expression, intracellular glutathione (GSH) levels, and HIV disease progression.
Main Methods:
- Multiparameter Flow Cytometry (FACS) analysis was employed.
- Simultaneous quantification of surface CD20 density and intracellular GSH levels in B lymphocytes.
Main Results:
- CD20 expression is significantly elevated on B lymphocytes from HIV-infected individuals.
- Elevated CD20 expression correlates with more advanced HIV disease.
- Intracellular GSH levels exhibit greater heterogeneity in HIV-infected individuals compared to controls.
- A positive correlation exists between intracellular GSH levels and CD20 expression on a per-cell basis.
Conclusions:
- CD20 expression on B cells is a potential surrogate marker for monitoring HIV infection.
- The observed changes in CD20 and GSH suggest altered B cell metabolism during HIV infection.