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Related Experiment Videos

CD45 isoform expression during T cell development in the thymus.

Y Fujii1, M Okumura, K Inada

  • 1First Department of Surgery, Osaka University Medical School, Japan.

European Journal of Immunology
|July 1, 1992
PubMed
Summary
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Human T cell development involves CD45 isoform switching. Early thymocytes express CD45RA, while mature T cells switch to CD45RO, with a final shift back to CD45RA in cord blood T cells.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Leukocyte common antigen (CD45) exists in various isoforms.
  • CD45 isoform expression differs across T cell developmental and activation stages.

Purpose of the Study:

  • To investigate CD45 isoform expression on human T cell subsets.
  • To elucidate the sequence of CD45 isoform switching during T cell development.

Main Methods:

  • Utilized two- and three-color flow cytometry.
  • Employed cell depletion techniques.
  • Analyzed CD45RA and CD45RO expression on thymocytes and cord blood T cells.

Main Results:

  • Double-negative thymocytes showed CD45RA-RO- or CD45RA+RO- expression.
  • Double-positive and most single-positive thymocytes expressed CD45RA-RO+.

Related Experiment Videos

  • Cord blood T cells predominantly expressed CD45RA+RO-.

    • Conclusions:

    • T cell development involves a sequential switch from CD45RA to CD45RO, followed by a return to CD45RA.
    • Single-positive thymocytes resemble double-positive cells more than cord blood T cells in CD45 isoform expression.
    • The final switch to CD45RA on cord blood T cells signifies a maturation step in the thymus.