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Related Experiment Videos

CD40 is functionally expressed on human thymic epithelial cells.

A H Galy1, H Spits

  • 1DNAX Research Institute, Department of Human Immunology, Palo Alto, CA 94304-1104.

Journal of Immunology (Baltimore, Md. : 1950)
|August 1, 1992
PubMed
Summary

CD40 is expressed on human thymic epithelial cells (TEC) and can be upregulated by inflammatory cytokines. Triggering CD40 on TEC promotes granulocyte-macrophage colony-stimulating factor secretion, indicating its functional role in the thymus.

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Area of Science:

  • Immunology
  • Cell Biology
  • Thymic Epithelial Cell Biology

Background:

  • CD40 is a B cell antigen also found on some epithelial cells and carcinomas.
  • Its distribution within the human thymus was previously uncharacterized.

Purpose of the Study:

  • To investigate the expression and function of CD40 on human thymic epithelial cells (TEC).

Main Methods:

  • Immunohistology to determine CD40 distribution in the thymus.
  • Culture of TEC and thymic fibroblasts to assess CD40 expression and regulation.
  • Northern blot analysis for CD40 mRNA.
  • Stimulation assays using anti-CD40 monoclonal antibodies (mAbs) and cytokines to evaluate functional responses.

Main Results:

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  • CD40 was expressed on cortical and medullary TEC, but not on thymocytes or fibroblasts.
  • IL-1 alpha, TNF-alpha, and IFN-gamma upregulated membrane CD40 expression on cultured TEC.
  • CD40 mRNA in TEC was upregulated by IL-1 and IFN-gamma.
  • Triggering CD40 on TEC with anti-CD40 mAb, along with IFN-gamma and IL-1, induced dose-dependent granulocyte-macrophage colony-stimulating factor secretion.

Conclusions:

  • CD40 is expressed on human TEC and is a functional molecule.
  • CD40 signaling in TEC can modulate cytokine secretion, suggesting a role in thymic immune regulation.