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Updated: Aug 9, 2026

Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes
Published on: January 20, 2014
Correlation between multi-headed spermatozoa in the ejaculate and histological findings in the testis
N Hofmann1, B Fervers, B Hilscher
1Section of Andrology, Heinrich-Heine-University, Düsseldorf, Germany.
Abstract:
Semen smears of 163 patients as well as bioptic material of 163 patients (93 cases of paraffin histology and 70 cases of cryostat sections) were grouped by their content of multi-headed spermatozoa or multi-nucleated spermatids, respectively. It could be demonstrated that findings in the ejaculate concerning multi-headed spermatozoa are in good agreement with those in the testis in respect of the presence of double- and multi-nucleated spermatids. Furthermore, it could be shown that the frequency of multi-headed spermatozoa is positively correlated to inflammatory processes either of immunologic origin or caused by micro-organisms.
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