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Isolation of antibodies to gelatin from antigen-antibody complex by proteolysis

R ARNON, M SELA

    Science (New York, N.Y.)
    |July 8, 1960
    PubMed

    Antibodies were isolated by digestion of the gelatin-antigelatin complex with collagenase and removal of the gelatin fragments by dialysis. The pure antibody could be precipitated anew by the antigen. Antibodies to tyrosylgelatin were isolated in a similar way, but in this case peptides containing the active antigenic sites remained bound to the antibody.

    Keywords:
    ANTIBODIES

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