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Updated: Aug 12, 2026

16:42
Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
Published on: November 25, 2010
Use of in vitro-transcribed RNAs as immobilized targets during RNA:RNA hybridization
1Department of Molecular Genetics and Biochemistry, Univ. of Pittsburgh Sch. of Med., PA 15261-2072.
Biotechniques
|July 1, 1992
Abstract:
We describe the use of in vitro-transcribed complementary RNAs as filter-bound targets during nuclear run-on analyses. Use of these single-stranded reagents in high-stringency RNA:RNA hybridizations increases signal-to-background hybridization seen using DNA targets and allows efficient measurement of transcriptional rates across genes in either direction.
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