Related Experiment Videos
Expression of CD7 on normal human myeloid progenitors
C Chabannon1, P Wood, B Torok-Storb
1Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
Journal of Immunology (Baltimore, Md. : 1950)
|September 15, 1992
Summary
Normal human bone marrow contains a minor population of CD7+/CD34+ cells. These cells are committed myeloid progenitors, indicating CD7 expression occurs during early myeloid differentiation, not just T cell development.
Area of Science:
- Immunology
- Hematology
- Cell Biology
Background:
- Biphenotypic leukemias co-express CD7 and CD34.
- CD7 is typically considered a T cell-restricted antigen.
- CD34 is known as the human stem cell antigen.
Purpose of the Study:
- Investigate the presence of CD7+/CD34+ cells in normal human bone marrow.
- Determine the differentiation potential of these cells.
- Clarify the role of CD7 expression in hematopoiesis.
Main Methods:
- Flow cytometry to identify CD7+/CD34+ cells.
- Colony-forming unit (CFU) assays in semi-solid media.
- Long-term marrow cultures to assess granulocyte production.
- Complement-mediated cytotoxic assays.
Main Results:
- A minor subpopulation of CD7+/CD34+ cells was identified in human bone marrow.
- These CD7+/CD34+ cells contained committed myeloid progenitors.
- Myeloid progenitor differentiation was confirmed through CFU assays and granulocyte production.
- CD7 expression on myeloid progenitors was validated via cytotoxic assays.
Conclusions:
- CD7 is expressed on early myeloid differentiation stages, not exclusively on T cells.
- The CD7+/CD34+ population in bone marrow represents committed myeloid progenitors.
- This finding challenges the traditional view of CD7 as a strict T cell marker.