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Published on: January 12, 2015
Rod opsin cDNA sequence from the sand goby (Pomatoschistus minutus) compared with those of other vertebrates
S N Archer1, J N Lythgoe, L Hall
1Department of Biochemistry, University of Bristol, U.K.
Abstract:
The absorbance spectra of rods from the sand goby were measured by using microspectrophotometry. Analysis of the averaged spectra shows that the rod visual pigment has a maximum absorbance (lambda max) at approximately 501 nm. A sand goby retinal cDNA library was constructed and then screened with a partial sand goby rod opsin clone obtained by the polymerase chain reaction (PCR). The screening of the library yielded a full length rod opsin clone. The cDNA sequence and deduced amino acid sequence of this clone are compared with those of other vertebrate rod opsins.
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