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Related Experiment Videos

Hyaluronan-binding protein in endothelial cell morphogenesis.

S D Banerjee1, B P Toole

  • 1Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, Massachusetts 02111.

The Journal of Cell Biology
|November 1, 1992
PubMed
Summary
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Hyaluronan-binding protein (HABP) is crucial for endothelial cell migration and capillary-like tubule formation. Blocking HABP with an antibody or HA fragments inhibits these vital cellular processes.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Angiogenesis Research

Background:

  • Hyaluronan (HA) interaction with endothelial cells influences their behavior.
  • A monoclonal antibody, mAb IVd4, identifies hyaluronan-binding protein (HABP).

Purpose of the Study:

  • To investigate the role of HABP in endothelial cell migration and capillary-like tubule formation.
  • To determine the effect of mAb IVd4 and HA fragments on endothelial cell behavior.

Main Methods:

  • Endothelial cell migration assays after monolayer wounding.
  • Capillary-like tubule formation assays in collagen and basement membrane gels.
  • Immunocytochemical localization of HABP on migrating cells.

Main Results:

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  • mAb IVd4 significantly inhibited endothelial cell migration.
  • HA hexasaccharide also demonstrated inhibitory effects on cell migration.
  • Both reagents suppressed the morphogenesis of capillary-like tubules.
  • HABP was localized to the cell membrane and lamellipodia of migrating cells.

Conclusions:

  • HABP, recognized by mAb IVd4, plays a significant role in endothelial cell migration.
  • HABP is also involved in the formation of capillary-like tubules.
  • Targeting HABP may offer therapeutic strategies for angiogenesis-related conditions.