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A patch-clamp study of Bacillus subtilis.

I Szabó1, V Petronilli, M Zoratti

  • 1CNR Unit for the Physiology of Mitochondria, Department of Biomedical Sciences, Padova, Italy.

Biochimica Et Biophysica Acta
|November 23, 1992
PubMed
Summary
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Membrane stretch activates high-conductance channels in Bacillus subtilis. These channels, possibly involved in osmotic protection, show cooperative behavior and voltage modulation.

Area of Science:

  • Microbiology
  • Biophysics
  • Cell Biology

Background:

  • Gram-positive bacteria like Bacillus subtilis possess complex cell envelopes.
  • Understanding membrane transport mechanisms is crucial for bacterial survival and function.
  • The role of mechanosensitive channels in bacterial envelope physiology is an area of active research.

Purpose of the Study:

  • To investigate the presence and characteristics of stretch-activated channels in Bacillus subtilis.
  • To elucidate the biophysical properties and potential functions of these channels.
  • To explore the involvement of these channels in bacterial osmotic regulation.

Main Methods:

  • Utilized patch-clamp electrophysiology on giant protoplasts of Bacillus subtilis.
  • Applied mechanical stress (suction) to the protoplast membrane.

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  • Recorded and analyzed membrane resistance and channel activity (conductance, open probability, voltage modulation).

    • Main Results:

    • Membrane stretch induced an initial drop in resistance, followed by the appearance of stretch-activated, voltage-modulated, high-conductance channels.
    • Channel open probability increased exponentially with suction and positive voltage, influenced by mean open and closed times.
    • Observed substate structure and cooperative behavior, suggesting a family of interacting pores.

    Conclusions:

    • Bacillus subtilis possesses a family of mechanosensitive channels with cooperative behavior.
    • These channels likely play a role in osmotic protection.
    • The pores may be components of an unidentified envelope apparatus with additional functions in intact bacteria.