Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CD59 molecule: a second ligand for CD2 in T cell adhesion.

M Deckert1, J Kubar, D Zoccola

  • 1INSERM U343, Faculté de Médecine, Nice, France.

European Journal of Immunology
|November 1, 1992
PubMed
Summary

The T cell molecule CD2 binds to CD59, a protein previously thought to be unrelated. This interaction influences T cell adhesion and activation, revealing a new pathway in immune cell communication.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Imbalanced expression of glutamate-glutamine cycle enzymes induced by human T-cell lymphotropic virus type 1 Tax protein in cultivated astrocytes.

Journal of virology·1996
Same author

[Thymic carcinoid tumor causing paraneoplastic Cushing syndrome. Diagnostic value of double-labelled tomoscintigraphy].

Presse medicale (Paris, France : 1983)·1996
Same author

Cytokines are increased in the rat hippocampus after serotonergic neuron degeneration and upregulate the expression of GDH, an enzyme involved in glutamate detoxification.

Journal of neuroimmunology·1996
Same author

Long-term toxicity of intravenous mercury injection.

Lancet (London, England)·1996
Same author

Increased Clara cell protein in serum of firefighters.

Clinical chemistry·1996
Same author

Selective isolation of transiently transfected cells from a mammalian cell population with vectors expressing a membrane anchored single-chain antibody.

Journal of immunological methods·1996

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Interactions

Background:

  • The T cell surface molecule CD2 plays a key role in cell adhesion and activation, primarily through its interaction with CD58 (LFA-3).
  • Emerging evidence suggests CD2 may interact with additional ligands beyond CD58.
  • Understanding CD2's ligand repertoire is crucial for deciphering T cell-mediated immune responses.

Purpose of the Study:

  • To investigate the potential binding interaction between the T cell molecule CD2 and the complement regulatory protein CD59.
  • To characterize the functional consequences of CD2-CD59 interaction on cell adhesion and T cell activation.

Main Methods:

  • Rosette formation assays using Chinese hamster ovary (CHO) cells expressing human CD59 or CD2, and human T cells or erythrocytes.

Related Experiment Videos

  • Inhibition studies utilizing monoclonal antibodies (mAbs) targeting CD2, CD59, and CD58.
  • Binding assays with radiolabeled CD59 molecules and CD2-expressing cells.
  • Analysis of T cell activation markers following CD59 binding.

    • Main Results:

    • CD59-expressing cells formed rosettes with T cells and CD2-expressing cells, which were inhibited by CD2 and CD59 mAbs.
    • Specific binding of CD59 to CD2-expressing cells was demonstrated and inhibited by unlabeled CD59 and CD2 mAbs.
    • Certain CD59 mAbs (YTH 53.1, MEM 43) enhanced rosette formation, indicating epitope-specific effects.
    • CD59 binding to T cells induced the expression of CD2 regulatory (CD2R) epitopes.

    Conclusions:

    • Direct evidence confirms that the T cell molecule CD2 binds to CD59.
    • This CD2-CD59 interaction represents a novel pathway influencing T cell adhesion and activation.
    • The binding induces subtle molecular changes on T cells, suggesting a role in modulating immune responses.