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Binding protein for 5 alpha-dihydrotestosterone in mouse submandibular gland
Biochimica Et Biophysica Acta
|January 24, 1977
Summary
In mice, a 5 alpha-dihydrotestosterone binding protein appears at birth. Male levels decrease with testosterone, but castration restores them, suggesting endogenous hormone occupies binding sites.
Area of Science:
- Endocrinology
- Developmental Biology
- Molecular Biology
Background:
- The submandibular gland undergoes androgen-independent development in early life.
- Hormone-binding proteins play crucial roles in regulating cellular responses to hormones.
Purpose of the Study:
- To investigate developmental changes in 5 alpha-dihydrotestosterone binding protein levels in mouse submandibular glands.
- To compare these changes between male and female mice.
- To elucidate the role of endogenous androgens in regulating these binding protein levels.
Main Methods:
- DEAE-cellulose filter assay was used to quantify the binding protein in cytoplasmic extracts.
- Levels were measured in male and female mice at different developmental stages.
- The effect of castration on binding protein levels in mature male mice was assessed.
Main Results:
- The binding protein was detectable in both sexes by 5 days postpartum.
- Female mice maintained consistent levels, while male levels decreased significantly after 3 weeks, coinciding with rising serum testosterone.
- Castration of mature males led to a rapid increase in binding protein levels, reaching those of females and immature mice.
Conclusions:
- The decrease in 5 alpha-dihydrotestosterone binding protein in mature male mice is likely due to the occupancy of binding sites by endogenous testosterone.
- This suggests a regulatory mechanism where high endogenous androgen levels reduce the capacity for exogenous hormone binding.