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Escherichia coli integration host factor stabilizes bacteriophage Mu repressor interactions with operator DNA in
R Alazard1, M Bétermier, M Chandler
1Centre de Recherches en Biochimie et Génétique Cellulaires, Toulouse, France.
Molecular Microbiology
|June 1, 1992
Abstract:
Using gel retardation and DNase I protection techniques, we have demonstrated that the Escherichia coli integration host factor (IHF) stabilizes the interaction between Mu repressor and its cognate operator-binding sites in vitro. These results are discussed in terms of a model in which IHF may commit the phage to the lytic or lysogenic pathway depending on the occupancy of the operator sites by the repressor.