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Related Experiment Videos

Microplate coagulation assays.

C W Pratt1, D M Monroe

  • 1Division of Hematology, University of North Carolina, Chapel Hill 27599-7035.

Biotechniques
|September 1, 1992
PubMed
Summary
This summary is machine-generated.

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New microplate assays enable precise blood coagulation testing. These methods offer reliable prothrombin time and activated partial thromboplastin time measurements for various diagnostic applications.

Area of Science:

  • Biochemistry
  • Hematology
  • Clinical Diagnostics

Background:

  • Traditional blood coagulation assays can be time-consuming and require larger sample volumes.
  • Developing rapid, miniaturized assays is crucial for efficient laboratory diagnostics.

Purpose of the Study:

  • To develop and validate microplate-based assays for blood coagulation testing.
  • To establish reliable methods for measuring prothrombin time (PT) and activated partial thromboplastin time (aPTT).

Main Methods:

  • Utilized a kinetic microplate reader to monitor absorbance changes at 405 nm during plasma clotting.
  • Developed standardized procedures for PT and aPTT assays in a microplate format.

Main Results:

  • Achieved low intra- and inter-assay variability (few percentage points) for both PT and aPTT.

Related Experiment Videos

  • Established normal ranges: PT 64.5 ± 3.6 s, aPTT 69.8 ± 3.2 s.
  • Demonstrated expected prolongation of clotting times with factor-deficient plasmas.

    • Conclusions:

    • Microplate-based coagulation assays provide a sensitive, efficient, and reproducible alternative to conventional methods.
    • These assays are adaptable for specific applications, including factor quantification and activity assessments.