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[Gastroenteritis caused by verotoxin-producing Escherichia coli O157. Presentation of 2 cases]
1Servicio de Microbiología y Parasitología, Hospital Nuestra Señora del Pino, Las Palmas de Gran Canaria.
Abstract:
Verotoxin-producing Escherichia coli strains have been associated with acute hemorrhagic colitis since 1982. We have systematically investigated this pathogen in our laboratory, in all stool samples submitted for culture during a fourteen month period, by using MacConkey sorbitol agar to isolate non sorbitol fermenting Escherichia coli strains. Coated latex particles with an antiserum against Escherichia coli O157 were used to detect O157 serogroup Escherichia coli. A completed serological study and verotoxin assay was performed in all positive strains. We have found two non-related cases of verotoxigenic Escherichia coli infection in two children. In one case, the main clinical picture was an acute hemorrhagic colitis and the other one was a diarrhea without presence of blood, with fever and vomiting. Both cases improved without antimicrobial treatment. No systemic complications appeared in any of the cases during the infection. The infection incidence was 0.07% of all positive stool cultures. The few documented cases of this infection in our country should encourage to investigate this pathogen in order to know its real incidence in our environment.
Insights
Verotoxin-producing Escherichia coli (VTEC) infections can cause severe illness, including acute hemorrhagic colitis. This study identified two pediatric cases, highlighting the need for further investigation into VTEC incidence.
Area of Science:
- Microbiology
- Infectious Diseases
- Gastroenterology
Background:
- Verotoxin-producing Escherichia coli (VTEC) strains are recognized pathogens linked to acute hemorrhagic colitis since 1982.
- Systematic laboratory investigation of stool samples is crucial for identifying VTEC infections.
Observation:
- MacConkey sorbitol agar was used to isolate non-sorbitol fermenting E. coli.
- Latex particle agglutination with O157 antiserum identified E. coli O157 serogroup.
- Serological studies and verotoxin assays confirmed positive strains.
Findings:
- Two unrelated pediatric cases of VTEC infection were identified.
- Clinical presentations ranged from acute hemorrhagic colitis to non-bloody diarrhea with fever and vomiting.
- Both children recovered without antimicrobial treatment, and no systemic complications occurred.
Implications:
- The infection incidence was found to be 0.07% of positive stool cultures.
- Limited documented cases in the country suggest underreporting.
- Further research is recommended to determine the true incidence and epidemiology of VTEC in the region.