Related Experiment Videos
Modified hemoglobin solution as possible perfusate relevant to organ transplantation
T Agishi1, K Sonda, I Nakajima
1Dept of Surgery, Tokyo Women's Medical College, Japan.
Summary
A modified hemoglobin solution, pyridoxylated hemoglobin-polyoxyethylene conjugate solution (PHP solution), shows promise for organ transplantation by extending preservation times for kidneys and livers. It also facilitates antibody removal, potentially aiding xenotransplantation.
Area of Science:
- Biomedical Engineering
- Transplantation Immunology
- Biochemistry
Background:
- Modified hemoglobin solutions offer acellular oxygen-carrying capacity.
- These solutions are being explored for organ preservation and transplantation applications.
Purpose of the Study:
- To evaluate the efficacy of a pyridoxylated hemoglobin-polyoxyethylene conjugate (PHP) solution in extending organ viability.
- To assess the potential of PHP solution in antibody removal for transplantation.
Main Methods:
- PHP solution was used to perfuse canine kidneys after 4.5 hours of warm ischemia.
- Canine livers were preserved with PHP solution for 48 hours.
- Rat small intestines were perfused with PHP solution for 12 hours and compared to controls.
- Exchange transfusion with PHP solution was performed on rats to assess antibody removal.
Main Results:
- Canine kidneys survived 4.5-hour warm ischemia followed by PHP perfusion.
- Canine livers survived 48-hour preservation with PHP solution.
- PHP solution perfusion of rat small intestines resulted in higher survival rates than Collins or UW solutions.
- PHP solution exchange transfusion significantly reduced IgG, IgA, and IgM levels in rats.
Conclusions:
- PHP solution demonstrates potential for extending organ preservation times in transplantation.
- PHP solution effectively removes antibodies, suggesting utility in xenotransplantation by reducing pre-formed antibodies.