[Oxidative phosphorylation of liver mitochondria in Oncomelania snail]

Oxidative phosphorylation of liver mitochondria in Oncomelania snail was separately detected by using oxygen electrode and spectrophotometer. ADP increased oxidative reaction of liver mitochondria from 0.187 to 0.318 mumol O2/mg protein.20 min. When certain substrates of citric acid cycle were added to liver mitochondria of Oncomelania snail, we found that oxidative phosphorylation increased to 0.353-0.444 mumol O2/mg protein.20 min. ATPase was detected in the liver of Oncomelania snail. The oxidative phosphorylation of mitochondria in Oncomelania snail could be markedly inhibited by DNP and molluscicide bromoacetamide, but the latter didn't show the inhibition of ATPase. (Figs. 1,2).