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Methods for the analysis of persistent chlorinated hydrocarbons in tissues
1Division of Chemistry and Toxicology, Virginia Institute of Marine Science, College of William and Mary, Gloucester Point 23062.
Journal of Chromatography
|September 16, 1992
Summary
Chlorinated hydrocarbons bioaccumulate in tissues, posing health risks. Advanced analytical techniques are crucial for accurate quantification and identification of these persistent organic pollutants in biological samples.
Area of Science:
- Environmental Chemistry
- Toxicology
- Analytical Chemistry
Background:
- Chlorinated hydrocarbons are persistent organic pollutants known to bioaccumulate in tissues.
- Exposure to these compounds can lead to severe health consequences.
- They exist as individual compounds, mixtures, or complex formulations.
Purpose of the Study:
- To review established and emerging methods for the extraction and analysis of chlorinated hydrocarbons in biological tissues.
- To highlight the challenges associated with accurately quantifying these compounds in complex matrices.
- To discuss advanced analytical techniques for congener-specific analysis and identification.
Main Methods:
- Tissue extraction and purification using conventional and advanced techniques like supercritical fluid extraction.
- Capillary gas chromatography for separation of complex mixtures.
- Sensitive and selective detection methods including electron-capture detection, electrolytic conductivity detection, and mass spectrometry.
- Congener-specific analysis and computer-assisted identification for accurate quantitation.
Main Results:
- Established extraction and purification schemes are available, with supercritical fluid extraction showing promise.
- Capillary gas chromatography coupled with sensitive detectors (ECD, ELCD, MS) is essential for analysis.
- Quantitation challenges arise from the complexity of mixtures and concentration variations in tissues.
- Congener-specific analysis and computer-assisted methods aid in overcoming these challenges.
Conclusions:
- Accurate analysis of chlorinated hydrocarbons in tissues requires sophisticated extraction and chromatographic techniques.
- Advanced detection and identification methods are critical for addressing quantitation difficulties.
- Understanding congener-specific concentrations is vital for assessing health risks associated with these bioaccumulative compounds.
