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Published on: September 10, 2017
Alpha-1 antitrypsin gene exon use in stimulated lymphocytes
M S Bashir1, K Morrison, D H Wright
1University Department of Pathology, South Block General Hospital, Southampton.
Aims:
To investigate the expression of mRNA transcripts containing exon A or B in lymphocyte cultures.
Methods:
An in situ hybridisation technique, using synthetic, biotinylated oligonucleotide probes was deployed to allow the demonstration of exon A, exon B, or the normal hepatocyte message containing exon C.
Results:
Lymphocytes used the same alternative splicing technique as monocytes in the generation of their alpha-1 antitrypsin message. They also provided data on the frequency of exon A and B expression in cells from different subjects. Most circulating granulocytes failed to show the alpha-1 antitrypsin message, suggesting that this protein is synthesised in the marrow and represents a stored protein component in polymorph and circulating nuclear lymphocytes.
Conclusions:
In situ hybridisation is a sensitive technique for the detection of individual gene exon use in cell populations. Lymphocytes show the same promoter use as that described for monocytes.
