[Activation of aerobic bacteria used as BOD measurement of seawater]

Waste of seawater used for cooling water for machines in some kinds of industries in a littoral district in Sakai City, comes under the application of BOD control of the pollution control ordinance in Osaka Prefecture. But unfortunately the BOD measurement method of seawater sample has not been established. In order to establish the measurement method, we studied the strength of activation of aerobic bacteria in the incubation water used and the dilution water. From our results it was proved that we can use the incubation seawater consisting of more than 10(3) cells/ml general bacteria count and more than 0.50 microgram/l ATP (Adenosin triphosphate) as the incubation water and the artificial seawater as the dilution water.