Related Experiment Video

Updated: Sep 5, 2026

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
07:27

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii

Published on: April 18, 2016

CONVERSION OF THE PHASE I ANTIGEN OF COXIELLA BURNETII TO HAPTEN BY PHENOL TREATMENT

R L ANACKER, W T HASKINS, D B LACKMAN

    Journal of Bacteriology
    |May 1, 1963
    PubMed

    Keywords:
    ANTIGEN-ANTIBODY REACTIONSCOMPLEMENT FIXATION TESTSCOXIELLAEXPERIMENTAL LAB STUDYGUINEA PIGSMICEPHENOLSPOLYSACCHARIDES, BACTERIALPRECIPITIN TESTSRABBITSTRICHLOROACETIC ACID

    More Related Videos

    Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants11:44

    Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants

    Published on: May 13, 2015

    Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing10:29

    Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing

    Published on: July 6, 2016

    Related Concept Videos

    Drug Metabolism: Phase I Reactions01:17

    Drug Metabolism: Phase I Reactions

    A phase I reaction is a biochemical process that introduces a functionally reactive polar group to a substance. This transformation predominantly occurs in the liver, facilitated by the cytochrome P450 system of hemoproteins situated in the lipophilic endoplasmic reticulum of cells. The metabolite generated through this process can have varying polarities. If it is sufficiently polar, it can be easily excreted in the urine due to its water compatibility. However, if the metabolite is nonpolar,...
    Oxidation of Phenols to Quinones01:17

    Oxidation of Phenols to Quinones

    In the presence of oxidizing agents, phenols are oxidized to quinones. Quinones can be easily reduced back to phenols using mild reducing agents. The electron-donating hydroxyl group enhances the reactivity of the aromatic ring, enabling oxidation of the ring even in the absence of an α hydrogen.
    o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox property is crucial in...
    Benzene to Phenol via Cumene: Hock Process01:27

    Benzene to Phenol via Cumene: Hock Process

    The synthesis of phenol from benzene via cumene and cumene hydroperoxide is called the Hock process. First, a Friedel–Crafts alkylation reaction of benzene with propene gives cumene. Then cumene forms cumene hydroperoxide via a radical chain reaction. In the chain initiation step, the benzylic hydrogen is abstracted to give a benzylic radical. In the chain propagation step, the benzylic radical reacts with an oxygen diradical to form a cumene hydroperoxide radical. The cumene hydroperoxide...
    Phase I Oxidative Reactions: Overview01:19

    Phase I Oxidative Reactions: Overview

    Phase I biotransformation, or functionalization, is a crucial chemical process that converts drugs and other xenobiotics into more water-soluble forms, facilitating expulsion from the body. It involves oxidative, reductive, and hydrolytic reactions that add or unveil polar functional groups on lipophilic substrates. Key players in phase I reactions are the mixed-function oxidases. Situated in liver cell microsomes, these enzymes predominantly carry out drug metabolism. They require molecular...
    Phase I Reactions: Reductive Reactions01:27

    Phase I Reactions: Reductive Reactions

    Phase I biotransformation reductive reactions are chemical processes that modify drugs by introducing or revealing polar functional groups via reduction. Enzymes called reductases catalyze these reactions, playing a pivotal role in drug metabolism by transforming lipophilic drugs into more polar, water-soluble metabolites for easy excretion. An essential type of reductive reaction is the carbonyl group reduction, where aldehydes and ketones are reduced to alcohols. An example is the...