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Solubility and diffusion coefficient of adenosine 3':5'-monophosphate.
The Journal of Biological Chemistry
|February 10, 1977
Summary
This study determined key physical parameters for adenosine 3':5'-monophosphate (cAMP). New values for molar extinction coefficient, aqueous solubility, and diffusion coefficient at pH 7.0 are now available.
Area of Science:
- Biochemistry
- Physical Chemistry
Background:
- Adenosine 3 ':5 '-monophosphate (cAMP) is a crucial second messenger.
- Accurate physical parameters are essential for quantitative biochemical studies.
Purpose of the Study:
- To determine previously unavailable physical parameters of cAMP.
- To provide essential data for researchers working with cAMP.
Main Methods:
- Spectrophotometry was used to determine the molar extinction coefficient.
- Aqueous solubility was measured at pH 7.0.
- Diffusion coefficient was determined using appropriate methods.
Main Results:
- The molar extinction coefficient of cAMP at pH 7.0 was found to be 1.38 X 10(-4).
- The aqueous solubility of cAMP at pH 7.0 is 0.0236 M.
- The diffusion coefficient of cAMP was determined to be 4.44 X 10(-6) cm2/s.
Conclusions:
- This research provides critical, previously unavailable physical data for cAMP.
- These parameters enhance the accuracy of quantitative cAMP research and biochemical assays.