Demonstration of sodium/calcium exchange in rodent osteoblasts

Based on the inhibition of stimulated Ca release from cultured bone by several different agents that alter Na transport, we proposed that hormonally stimulated bone resorption requires Na/Ca exchange. Calcemic hormones appear to interact primarily directly with the osteoblast, which then mediates the activation of osteoclast activity. In organ culture it is not possible to determine whether Na/Ca exchange is involved in this initiating step in the osteoblast or directly in osteoclast-mediated Ca release, and there have been no prior direct measurements of Na/Ca exchange in bone or bone cells. The purpose of this study was to demonstrate the presence of Na/Ca exchange transport in the osteoblast. Thus, we characterized Na-dependent Ca transport in osteoblast-like rat osteosarcoma cells (UMR-106) and primary bone cells isolated from neonatal mouse calvaria. Cells were loaded with the Ca-sensitive dye fura-2 in the presence of physiologic NaCl and the absence of Ca with or without 0.3 mM ouabain. Changes in free cytosolic Ca after the extracellular addition of 1.5 mM Ca were measured spectrofluorimetrically. An outward Na gradient was generated by decreasing extracellular Na while maintaining isotonicity. UMR-106 cells that were Na loaded by ouabain inhibition of Na,K-ATPase activity exhibited 30% greater Ca uptake than control cells. Similar results were obtained with primary bone cells. This uptake required extracellular Ca, was not inhibited by 200 microM verapamil, and was reversible upon reversal of the Na gradient. These data demonstrate the presence of a Na/Ca exchange transport system in osteoblasts.