A technique for segmental rectal and colonic perfusion in humans

Area of Science:

• Gastroenterology
• Colorectal Research
• Biomedical Engineering

Background:

• Characterizing pathophysiologic processes in the colon and rectum requires effective methods for collecting biological samples.
• Existing techniques may not adequately capture soluble substances and cells from standardized intestinal segments.

Purpose of the Study:

• To develop and validate a novel endoscopic perfusion technique for collecting soluble substances and cells from standardized rectal and sigmoid colon segments.
• To assess the safety, feasibility, and utility of this technique in healthy volunteers.

Main Methods:

• An endoscopic tube with inflatable balloons was used to isolate rectal and sigmoid colon segments.
• Segments were perfused with a buffer at 37°C, and perfusate/cells were collected.
• Fluoroscopic guidance ensured accurate tube placement and segment isolation.

Main Results:

• The technique was successfully completed in 45 out of 51 attempts with negligible leakage.
• Perfusate analysis in 18 healthy volunteers revealed measurable concentrations of albumin, eosinophil cationic protein, prostaglandin E2, and hyaluronan.
• Steady-state conditions were achieved by the second perfusion period, with perfusate containing primarily epithelial cells.

Conclusions:

• The developed perfusion technique is safe, well-tolerated by subjects, and provides quantitative data on soluble substances and cells.
• This method is a valuable tool for investigating colorectal inflammation and related pathophysiologic processes.