ISOLATION AND CHARACTERIZATION OF BACTERIOPHAGES ACTIVE ON CLOSTRIDIUM SPOROGENES

Betz, John V. (St. Bonaventure University, St. Bonaventure, N.Y.), and Kenneth E. Anderson. Isolation and characterization of bacteriophages active on Clostridium sporogenes. J. Bacteriol. 87:408-415. 1964.-Twelve bacteriophages active on the anaerobic species Clostridium sporogenes were studied. Four of these were isolated by the authors, and eight were obtained from L. S. McClung of Indiana University. The 12 phages studied could be distinguished into three groups on the basis of their plaque morphology, host range, receptor sites, and serological relationships. One group contained ten of the phages which were serologically related. These were separated into three subgroups on the basis of plaque morphology, host range, and receptor sites. The heat sensitivities of four phages were correlated with their classification in this scheme. Of 25 strains of C. sporogenes tested for lysogenicity, none was found to be lysogenic, but 9 produced bacteriocin-like substances and 20 were sensitive to one or more of these.