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Innovative endpoint determination system for antifungal susceptibility testing of yeasts
R Tellier1, M Krajden, G A Grigoriew
1Department of Microbiology, Toronto Hospital, Ontario, Canada.
Antimicrobial Agents and Chemotherapy
|August 1, 1992
Abstract:
Fungal infections and antifungal resistance are increasingly recognized. Antifungal susceptibility testing remains unstandardized, and a particularly important problem is endpoint determination. In this paper we propose the yeast metabolic reduction of the tetrazolium salt 2,3-bis(2- methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetraz olium hydroxide (XTT) as a colorimetric endpoint which is quantitative and objective. Amphotericin B, fluorocytosine, and fluconazole dose-response curves were obtained, and a metabolic MIC could be determined by using precise criteria.