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Pseudomonas aeruginosa biofilm as a diffusion barrier to piperacillin
B D Hoyle1, J Alcantara, J W Costerton
1Department of Biological Sciences, University of Calgary, Alberta, Canada.
Antimicrobial Agents and Chemotherapy
|September 1, 1992
Abstract:
Pseudomonas aeruginosa 579 biofilms formed on dialysis membranes retarded piperacillin diffusion. Treatment of biofilms with 5.0 mM CaCl2.2H2O prevented diffusion. Biofilms permitted equilibration of [14C]glucose. Thin-layer chromatography of fluids distal to untreated (viable and nonviable) and viable Ca(2+)-treated P. aeruginosa 579 biofilms and fluids distal to a viable P. aeruginosa mutant noninducible for the expression of beta-lactamase did not detect piperacillinoic acid.