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Use of derivatization reactions with adsorptive stripping voltammetry for determining fotemustine in biological
M M Díaz De Guereñu1, R J Barrio, A Arranz
1Department of Analytical Chemistry, Faculty of Pharmacy, University of the Basque Country, Vitoria-Gasteiz, Spain.
Abstract:
A method is described for determining the new fotemustine antineoplastic in human serum. The method is based on the derivatization of the original molecule by means of diazotization and coupling reactions. The derivatization product is determined by means of adsorptive stripping voltammetry. The calibration graphs were linear over the range 6 x 10(-9)-8 x 10(-7) and 6 x 10(-10)-8 x 10(-8) M, according to whether the coupling reagent was 1-naphthylamine or 1-naphthol, respectively. At the same time, the detection limits are 4.1 x 10(-9) and 1.4 x 10(-10) M, respectively. The method was applied to determine this antineoplastic in human serum, after a liquid-liquid extraction process, from which recovery factors of 95.6% has been obtained.