Related Experiment Video
Updated: Jun 14, 2026

05:55
Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System
Published on: December 21, 2019
N6-BENZYLADENINE: INHIBITOR OF RESPIRATORY KINASES
Summary
N(4)-benzyladenine, a plant hormone, slows down respiration and extends the shelf life of vegetables. This effect may be due to its competitive inhibition of glycolytic kinases, crucial enzymes in energy production.
Area of Science:
- Plant Physiology
- Biochemistry
- Horticultural Science
Background:
- N(4)-benzyladenine is an active cytokinin known to affect plant processes.
- Cytokinins play vital roles in plant growth, development, and senescence.
Purpose of the Study:
- To investigate the mechanism by which N(4)-benzyladenine inhibits respiration in plant explants.
- To explore the potential link between N(4)-benzyladenine's effects and glycolytic kinase activity.
Main Methods:
- Experiments were conducted on various plant explants.
- Respiration rates were measured in the presence of N(4)-benzyladenine.
- Enzyme kinetics, specifically competitive inhibition of glycolytic kinases, were investigated.
Main Results:
- N(4)-benzyladenine was observed to inhibit the respiration of multiple plant explants.
- The plant hormone demonstrated a senescence-delaying effect, extending postharvest life of leafy vegetables.
- Evidence suggests N(4)-benzyladenine competitively inhibits glycolytic kinases.
Conclusions:
- N(4)-benzyladenine's inhibition of respiration and delay of senescence may be mediated by competitive inhibition of glycolytic kinases.
- This mechanism provides insight into the physiological effects of N(4)-benzyladenine on postharvest quality of vegetables.
Related Concept Videos
Enzyme Inhibition
Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
The Electron Transport Chain
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q in...
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q in...
Indirect-Acting Cholinergic Agonists: Chemistry and Structure-Activity Relationship
Indirect-acting cholinergic agonists are agents that interact with the acetylcholinesterase enzyme in the synaptic cleft, preventing the breakdown of acetylcholine into choline and acetate. Consequently, the concentration of acetylcholine in the synaptic cleft increases. These agonists can be classified into reversible and irreversible inhibitors based on their duration of action.
Reversible inhibitors display short to medium durations of action. Short-acting agents include simple alcohols with...
Reversible inhibitors display short to medium durations of action. Short-acting agents include simple alcohols with...
Indirect-Acting Cholinergic Agonists: Mechanism of Action
Indirect-acting cholinergic agonists work by interacting with an enzyme called acetylcholinesterase (AChE) in the synaptic cleft. They can be reversible or irreversible inhibitors and have different effects on the enzyme.
Reversible inhibitors like edrophonium bind to a specific part of the enzyme called the anionic catalytic site. They form noncovalent bonds, which means they are not strongly attached to the enzyme. This creates a temporary and less stable enzyme–inhibitor complex, leading to...
Reversible inhibitors like edrophonium bind to a specific part of the enzyme called the anionic catalytic site. They form noncovalent bonds, which means they are not strongly attached to the enzyme. This creates a temporary and less stable enzyme–inhibitor complex, leading to...
Depolarizing Blockers: Mechanism of Action
Depolarizing blockers act on skeletal muscle fibers' membranes and induce their depolarization. Most depolarizing blockers have two quaternary N+ atoms that bind the nicotinic acetylcholine receptors and cause neuromuscular blockade within minutes.
Succinylcholine is the most commonly used depolarizing blocker. Chemically, it constitutes two molecules of acetylcholine joined together by an acetate methyl group. They act on the receptors in the same way as acetylcholine. Because succinylcholine...
Succinylcholine is the most commonly used depolarizing blocker. Chemically, it constitutes two molecules of acetylcholine joined together by an acetate methyl group. They act on the receptors in the same way as acetylcholine. Because succinylcholine...
Depolarizing Blockers: Pharmocokinetics
Depolarizing blockers are administered through intravenous injection. Succinylcholine is the most common choice of depolarizing blockers in emergency clinical practices. Although they have a rapid onset, they readily diffuse away from the motor end plate into the extracellular fluid. They are metabolized by enzymes such as liver butyrylcholinesterase and plasma pseudocholinesterases. This produces a short duration of action, typically 5-10 minutes long, unlike nondepolarizing blockers, which...

