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SOME CHARACTERISTICS OF AN OLEATE-REQUIRING, HEMOLYTIC PEDIOCOCCUS

R H DEIBEL, J H SILLIKER, P T FAGAN

    Journal of Bacteriology
    |October 1, 1964
    PubMed

    Insights

    A novel hemolytic Pediococcus requiring oleic acid was identified. This oleate-dependent hemolysis differs from typical Pediococcus characteristics and was not observed in other patient samples.

    Area of Science:

    • Microbiology
    • Bacteriology

    Background:

    • Pediococcus species are generally known for their fermentation capabilities.
    • Limited information exists on Pediococcus strains with specific nutritional requirements or hemolytic activity.

    Purpose of the Study:

    • To describe the characteristics of a unique, oleic acid-requiring, hemolytic Pediococcus.
    • To investigate the nutritional and hemolytic properties of this novel bacterial isolate.

    Main Methods:

    • Isolation and characterization of a gram-positive, catalase-negative, tetrad-forming coccus from a bronchial aspirate.
    • Growth studies to determine nutritional requirements, specifically oleic acid.
    • Hemolysis assays using blood agar with varying oleic acid supplementation.

    Main Results:

    • The isolate, classified as Pediococcus, demonstrated an unusual requirement for oleic acid for growth, even in complex media.
    • Hemolysis was observed on specific human blood agar formulations and was dependent on oleate supplementation.
    • The oleic acid concentration required for hemolysis exceeded that for growth, and no cell-free hemolysin was detected.

    Conclusions:

    • This study describes a novel Pediococcus strain with a distinct oleic acid requirement and hemolytic capability.
    • The findings highlight the variability within the Pediococcus genus and the specific conditions influencing its hemolytic activity.

    Keywords:
    CULTURE MEDIAEXPERIMENTAL LAB STUDYOLEIC ACIDPEDIOCOCCUSSPUTUMTUBERCULOSIS

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