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ICMSF Methods Studies. XI. Collaborative/Comparative Studies on Determination of Coliforms Using the Most Probable
J H Silliker1, D A Gabis1, A May1
1Silliker Laboratories, 1304 Halsted Street, Chicago Heights, Illinois 60411 and The Pillsbury Company, Minneapolis, Minnesota.
Journal of Food Protection
|March 1, 2019
Summary
Two studies evaluated the Most Probable Number (MPN) technique for coliform detection in food. Variations between labs were significant, but differences between media were smaller, impacting food safety monitoring.
Area of Science:
- Food Microbiology
- Analytical Chemistry
Background:
- Accurate coliform enumeration is crucial for food safety.
- The Most Probable Number (MPN) technique is widely used but requires validation.
Purpose of the Study:
- To compare different MPN methods for coliform determination in foods.
- To assess variability in coliform testing across laboratories and media.
Main Methods:
- Two international collaborative studies were conducted.
- Three different presumptive and confirmatory media were evaluated.
- Inoculated and naturally contaminated foods were tested across multiple laboratories.
Main Results:
- Significant inter-laboratory variation (up to 3.3 log units) was observed.
- Differences between confirmatory media were minimal (0.2-0.5 log units).
- Replicate aliquot variation was a key source of intra-laboratory error.
Conclusions:
- MPN technique performance varies significantly between laboratories.
- Media choice has a lesser impact on coliform enumeration results compared to laboratory variability.
- Findings highlight the need for standardized protocols in microbiological testing for food safety regulations.
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