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Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
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Published on: May 4, 2012

ANTIBODIES AGAINST THE COMPONENT POLYPEPTIDE CHAINS OF BOVINE INSULIN

Y YAGI, P MAIER, D PRESSMAN

    Science (New York, N.Y.)
    |February 5, 1965
    PubMed

    Antibodies were produced in guinea pigs against A and B chains of bovine insulin; the chains were prepared by cleavage of all the disulfide bonds with sulfite. Antibodies against A chain reacted with A chain, but not with B chain or native insulin. Antibodies against B chain reacted strongly with both B chain and insulin, but not with A chain. Antibodies against insulin reacted strongly with insulin, weakly with B chain, and not at all with A chain. The results indicate that the insulin-reactive antibodies in antiserums to B chain and to insulin differ and are directed to differing individual antigenic regions of the insulin molecule.

    Keywords:
    CATTLEEXPERIMENTAL LAB STUDYGUINEA PIGSINSULIN ANTIBODIESPEPTIDES

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