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Related Experiment Videos

Freezing cocultured human blastocysts.

Y Ménézo1, B Nicollet, N Herbaut

  • 1Institut Rhonalpin pour l'Etude de la Reproduction Humaine/Fondation Mérieux, Bron, France.

Fertility and Sterility
|November 1, 1992
PubMed
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Freezing cocultured human blastocysts can achieve good pregnancy rates (PRs). This method offers an alternative for patients experiencing repeated in vitro fertilization failures.

Area of Science:

  • Reproductive Medicine
  • Embryology
  • Cryobiology

Background:

  • In vitro fertilization (IVF) programs generate supernumerary embryos.
  • Cryopreservation of embryos, including blastocysts, is a standard practice.
  • Optimizing freezing and thawing protocols is crucial for successful embryo transfer.

Purpose of the Study:

  • To evaluate the feasibility of achieving successful pregnancies after cryopreservation of cocultured human blastocysts.
  • To compare different freezing protocols for human blastocysts.

Main Methods:

  • Human blastocysts from IVF patients were frozen using three distinct protocols.
  • One protocol involved sucrose addition and simplified preparation steps.
  • Pregnancy rates (PRs) and ongoing pregnancies after embryo transfer (ET) were assessed.

Related Experiment Videos

Main Results:

  • A protocol utilizing sucrose and reduced preparation steps yielded a 21% PR per transfer.
  • This resulted in 15 ongoing pregnancies from 101 embryo transfers.
  • The study involved the thawing and transfer of 106 blastocysts.

Conclusions:

  • Cryopreservation of cocultured human blastocysts is a viable option for achieving good pregnancy rates.
  • This technique provides an alternative for patients with recurrent implantation failures.
  • Successful blastocyst freezing can improve IVF outcomes and reduce treatment cycles.