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Crystallization and preliminary X-ray analysis of the complex between a mouse Fab fragment and a single IgG-binding
J P Derrick1, G J Davies, Z Dauter
1Department of Biochemistry, University of Leicester, U.K.
Journal of Molecular Biology
|October 20, 1992
Abstract:
The Fab fragment of a mouse immunoglobulin G1, complexed with a single IgG-binding domain from streptococcal protein G, has been crystallized in a form suitable for analysis by X-ray diffraction. The needle-shaped crystals were grown from polyethylene glycol 4000 using vapour diffusion methods and diffract to 2.3 A resolution. The space group is P2(1)2(1)2(1) (a = 64.5 A, b = 70.5 A and c = 120.1 A), with one Fab-protein G domain complex in the asymmetric unit. Solution of the three-dimensional structure of the complex will permit a detailed analysis of the molecular interactions between protein G and the Fab portion of IgG.