Simple, efficient and simultaneous determination of anabolic steroids in human urine

A high performance liquid chromatographic method for the determination of free anabolic drug "sustanon 250" (Organon) is developed. Four testosterone esters, propionate, phenylpropionate, isocaproate and decanoate were detected simultaneously and quantitatively in only 15 minutes. The percentage extraction in dichloromethane of the four testosterone esters from doped urine was 88, 87, 89 and 94% respectively. The separation recorded at 254 nm using analytical column ODS-C18 and methanol as an eluent, showed no interference of naturally occurring steroids. This method can be used for pharmacokinetic studies, routine analysis in pharmaceutical industry, routine therapeutic surveillance and in drug abuse by sportsmen.