TLC determination of iodochlorhydroxyquin and its conjugate in plasma

A simple, specific, reliable, and sensitive method for the determination of iodochlorhydroxyquin and/or its conjugate in biological fluids is described. The method is based on a quantitative ether-acetone (1:1) extraction of plasma samples followed by TLC separation, visualization, elution, and determination at 267 nm. Iodochlorhydroxyquin released by hydrolysis of its conjugate was analyzed. Both compounds are detectable in amounts as low as 0.04 microgram/ml. Application of the one-compartment open model to the data (assuming the biotransformation of the drug in conjugated form) provides a pharmacokinetic profile for the 50-mg/kg dose of iodochlorhydroxyquin in Wistar male rats.