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Related Experiment Videos

ELISA test, a new method to detect and quantify isoproturon in soil.

E Liégeois1, Y Dehon, B de Brabant

  • 1C.R.U.P.A. Sérologie, I.R.S.I.A., Unité de Microbiologie Faculté des Sciences Agronomiques, Gembloux, Belgium.

The Science of the Total Environment
|August 12, 1992
PubMed
Summary

A new Enzyme-Linked ImmunoSorbent Assay (ELISA) accurately quantifies the herbicide isoproturon in soil. This method, using specific monoclonal antibodies, offers a reliable alternative to gas chromatography for soil residue analysis.

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Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry
  • Immunochemistry

Background:

  • Phenylurea herbicides like isoproturon are widely used in agriculture.
  • Accurate quantification of herbicide residues in soil is crucial for environmental monitoring.
  • Existing analytical methods may have limitations in sensitivity or practicality.

Purpose of the Study:

  • To develop and validate an indirect competitive Enzyme-Linked ImmunoSorbent Assay (ELISA) for quantifying isoproturon in soil samples.
  • To assess the reliability and cross-reactivity of the developed ELISA method.
  • To compare ELISA results with established gas chromatography methods.

Main Methods:

  • Production of specific monoclonal antibodies (MAbs) against isoproturon using a conjugated immunogen.

Related Experiment Videos

  • Development of an indirect competitive ELISA protocol utilizing the selected MAb.
  • Extraction of isoproturon from soil samples using methanol (total residue) and CaCl2 (soil solution availability).
  • Simultaneous analysis of soil extracts by both ELISA and gas chromatography (GC).

    • Main Results:

    • The developed ELISA successfully detected low concentrations of isoproturon (20-250 µg L⁻¹).
    • Cross-reactivity with other phenylurea herbicides was found to be insignificant.
    • High correlation coefficients (≥93.5%) were observed between ELISA and GC methods when using soil-spiked standard curves.
    • Soil organic matter and ionic strength were identified as interfering factors, addressed by using soil-specific standard curves.

    Conclusions:

    • The developed indirect competitive ELISA is a sensitive and specific method for quantifying isoproturon in soil.
    • ELISA provides a reliable and comparable alternative to gas chromatography for soil herbicide analysis.
    • The method's accuracy is enhanced by establishing standard curves using soil extracts from the target matrix.