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Related Experiment Videos

Cell cultures from cryopreserved human lung tissue.

M Roth1, M Solèr, M Hornung

  • 1Department of Research and of Internal Medicine, University Hospital Basel, Switzerland.

Tissue & Cell
|January 1, 1992
PubMed
Summary

Cryopreservation of human lung biopsies in liquid nitrogen allows long-term storage. Fibroblasts derived from cryopreserved tissue maintain biological responsiveness, enabling future cell culture and research.

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Area of Science:

  • Biomedical Science
  • Cell Biology
  • Cryobiology

Background:

  • Primary human fibroblasts are crucial for studying gene induction.
  • Established methods for lung tissue preservation are limited.
  • Long-term storage of viable lung tissue is needed for cell line establishment.

Purpose of the Study:

  • To develop and validate a cryopreservation method for human lung biopsies.
  • To assess the biological characteristics of fibroblasts derived from cryopreserved lung tissue.
  • To determine if cryopreservation impacts cellular responsiveness.

Main Methods:

  • Human lung biopsies were cryopreserved in liquid nitrogen after a two-step cooling process.
  • Fibroblast cell cultures were generated from both fresh and cryopreserved lung tissues.

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  • Cell proliferation rates and responses to platelet-derived growth factor-BB (PDGF-BB) were compared.

    • Main Results:

    • Cryopreserved lung biopsies remained viable for over a year.
    • Fibroblasts from cryopreserved tissue showed no significant difference in doubling rates compared to fresh tissue.
    • PDGF-BB equally enhanced DNA synthesis in fibroblasts from both fresh and cryopreserved samples.
    • Macrophages, dendritic cells, and endothelial cells were also successfully recovered.

    Conclusions:

    • A reliable method for cryopreserving human lung biopsies has been established.
    • Cryopreservation preserves the biological characteristics and responsiveness of primary lung fibroblasts.
    • This technique enables long-term storage and future establishment of cell lines from lung tissue without compromising cellular integrity.