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[Possibilities for using bedside cards as secondary comparison in trace element studies with the PCR technique]
A Du Chesne1, U Schütte, B Brinkmann
1Institut für Rechtsmedizin, Westfälischen Wilhelms-Universität Münster.
Archiv Fur Kriminologie
|September 1, 1992
Summary
Bedside cards from medical records can serve as viable blood controls in forensic stain investigations. Even up to 10 years old, these cards yield recognizable DNA patterns using PCR methods for identification.
Area of Science:
- Forensic Science
- Genetics
- Biochemistry
Background:
- Forensic investigations often require control blood samples for stain analysis.
- Fresh blood samples may not always be available, necessitating alternative controls.
- Previous studies suggest various biological materials can be used as controls.
Observation:
- This study explored the utility of bedside cards from medical records as control blood samples.
- A stain investigation was conducted 7 months post-mortem using a bedside card.
- Ten bedside cards, some up to 9 years old, were analyzed using PCR.
Findings:
- Recognizable DNA patterns were obtained from SE 33 and TC 11 STR systems on bedside cards.
- Matching DNA profiles were identified across different sections (anti-A, anti-B, anti-AB, anti-D) of the cards.
- PCR analysis of bedside cards up to 10 years old proved effective for stain investigations.
Implications:
- Bedside cards offer a valuable, accessible alternative control blood source in forensic casework.
- This method enhances the reliability of stain investigations when fresh samples are unavailable.
- Extends the potential use of archived medical materials in forensic DNA analysis.