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The vacuolating virus of monkeys. I. Isolation, growth characteristics, and inclusion body formation

G D HSIUNG, W H GAYLORD

    The Journal of Experimental Medicine
    |December 1, 1961
    PubMed

    Insights

    A vacuolating virus from patas monkeys is serologically identical to SV40, a simian virus. X-ray treatment enhanced detection of this virus in patas cells, aiding further research.

    Area of Science:

    • Virology
    • Cell Biology
    • Primate Virology

    Background:

    • A novel vacuolating virus was isolated from patas monkey kidney cell cultures.
    • This virus was found to be serologically identical to Simian Virus 40 (SV40).
    • SV40 is a virus previously associated with rhesus and cynomolgus monkeys.

    Purpose of the Study:

    • To characterize the newly isolated vacuolating virus.
    • To determine the relationship between the patas virus and SV40.
    • To investigate methods for detecting and studying the virus in cell cultures.

    Main Methods:

    • Serological comparison of the isolated virus with known SV40 strains.
    • Infection of various cell types (patas, rhesus, human) with the virus.
    • X-ray treatment of cell cultures to enhance virus detection.
    • Microscopic examination for intranuclear inclusions and cytoplasmic vacuoles.
    • Co-infection experiments with poliovirus.

    Main Results:

    • The isolated patas virus was confirmed to be serologically identical to SV40.
    • X-ray treatment significantly facilitated the detection of the virus in patas cells.
    • Rhesus monkey and human cells showed relative insusceptibility but allowed long-term viral persistence.
    • Intranuclear inclusions appeared 2-3 days before cytoplasmic vacuoles in infected patas cells.
    • Previous PA-57 virus infection did not impact poliovirus yields, and doubly infected cells were distinguishable.

    Conclusions:

    • The vacuolating virus from patas monkeys is identical to SV40.
    • X-ray treatment is a valuable method for enhancing SV40 detection in specific cell lines.
    • Differential cellular susceptibility and distinct cytopathic effects aid in studying SV40 infection.
    • SV40 can persist in cells that are relatively insusceptible to its lytic effects.
    Keywords:
    MONKEYS/diseasesVIRUS DISEASES/experimental

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