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Characterization of bovine heparin-binding neurotrophic factor (HBNF): assignment of disulfide bonds
1Ludwig Institute for Cancer Research (Melbourne Branch), Parkville, Victoria, Australia.
Biochemistry International
|October 1, 1992
Abstract:
The topology of the disulfides in native heparin-binding neurotrophic factor (HBNF) isolated from bovine brain was studied by proteolytic digestion using trypsin, Asp-N endoproteinase and chymotrypsin and peptide mapping. Disulfide-linked peptides were identified by automated Edman degradation. It has been shown that there are disulfide bonds between Cys15-Cys44, Cys23-Cys53, Cys30-Cys57, Cys67-Cys99 and Cys77-Cys109.