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Nucleotide sequence of bovine interleukin-6 cDNA
L Droogmans1, I Cludts, Y Cleuter
1Laboratoire de Chimie Biologique, Université Libre de Bruxelles, Rhode-St-Genèse, Belgium.
DNA Sequence : the Journal of DNA Sequencing and Mapping
|January 1, 1992
Summary
Researchers cloned bovine interleukin-6 (IL-6) cDNA from a tumor, revealing a protein with significant homology to IL-6 in other species. This finding is crucial for understanding IL-6
Area of Science:
- Veterinary Medicine
- Molecular Biology
- Immunology
Background:
- Bovine leukemia virus (BLV) causes B cell lymphosarcoma in cattle.
- Interleukin-6 (IL-6) is a key cytokine involved in immune responses and inflammation.
- Understanding IL-6 in bovine diseases is important for animal health.
Purpose of the Study:
- To clone the complementary DNA (cDNA) encoding bovine interleukin-6 (IL-6).
- To analyze the genetic sequence and homology of bovine IL-6.
- To discuss the role of IL-6 in BLV-induced tumors.
Main Methods:
- Isolation of cDNA from a bovine-leukemia virus (BLV)-induced B cell-lymphosarcoma library.
- Cloning using bacteriophage lambda gt11.
- Sequence analysis of the cloned IL-6 cDNA.
Main Results:
- Successfully cloned the full-length bovine IL-6 cDNA.
- The encoded protein consists of 208 amino acids.
- Bovine IL-6 shares 65%, 53%, 42%, and 42% homology with porcine, human, mouse, and rat IL-6, respectively.
Conclusions:
- The cloning of bovine IL-6 provides a valuable tool for further research.
- The homology suggests conserved functions across species.
- Further investigation into IL-6's role in BLV pathogenesis is warranted.